Projektnummer

0050558

Zusammenfassung

A novel experimental design will be developed combining the biochemi- cal synthesis of customised model proteins, and the tailoring of artificial nano-cavities for single protein molecules regarding their geometric dimensions and the chemical nature of their walls. A set of dually labelled model proteins will be synthesised by native chemical ligation and expressed protein ligation methods. Their single pair fluorescence resonance energy transfer during folding within the cavities will be probed. By systematic variation of the cavity size two different problems will be addressed: in po...