Projektnummer

0051385

Zusammenfassung

Many cellular processes require allosteric proteins which contain at least two spatially separated substrate or effector binding sites with interdependent activities. Despite a large body of crystal structures there is no general knowledge about the mechanics and energetics of information transmission within such proteins. It is proposed to establish such principles from the molecular analysis of information transmission in Tet repressor (TetR), a well described tetracycline (tc) dependent regulator of transcription. TetR variants with different allostery and a peptide inducer, trigger...