Projektnummer

0051565

Zusammenfassung

Pleckstrin homology (PH) domains play a major role in lipid-mediated protein translocation and signaling events in proteins. Pleckstrin itself becomes activated upon phosphorylation by protein kinase C (PKC). During the previous funding period a FRET-based sensor (KCP-1) was developed that was able to monitor PKC activity in living cells. However, the structural basis for the conformational alterations linked to the FRET changes still needs to be demonstrated. In order to optimize KCP-1 for NMR-studies and its perfomance in cells, a reduction in size and an improvement of the FRET chan...