Projektnummer

0068858

Zusammenfassung

The current paradigm of nucleic acid polymerase reactions is that DNA synthesis is exclusively initiated from antiparallel-oriented template primer duplexes, yet new XNA-based experiments hint at the possibility that some polymerases are flexible enough to copy a natural nucleic acid template in the reverse direction and/or initiate from a 5' end from a parallel-hybridized primer. This project addresses the question whether polymerases are able to accept a non-canonical parallel-stranded primer template complex as a substrate to initiate nucleic acid synthesis. Such a previously undete...